Bacteriophage therapy for Acinetobacter baumannii-induced superficial wounds in experimental mice.

Mahmoud, Essam Saad; Taha, Reda; Bassyouni, Rasha H; Ahmed, Fatma A; Samhan, Farag A

doi:10.21608/ifjsis.2023.229869.1038

Bacteriophage therapy for Acinetobacter baumannii-induced superficial wounds in experimental mice.

Document Type : Original full papers (regular papers)

Authors

¹ Botany Department, Science Faculty, Fayoum University

² Botany Department, Faculty of Science, Fayoum University, Fayoum, Egypt.

³ Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Egypt.

⁴ Water and Wastewater Microbiology Department, National Research Centre, Dokki, Cairo, Egypt.

10.21608/ifjsis.2023.229869.1038

Abstract

Antibiotic resistance is one of the greatest hazards to human health in the modern era. Incredibly even more concerning is how quickly antibiotic-resistant bacteria can spread throughout the global community and in healthcare settings. In this study, locally isolated bacteriophage that has the ability to lyse multidrug-resistant bacteria was isolated from wastewater as one strategy to combat multidrug-resistant bacteria. Examination of phage morphology by electron microscope showed three-tailed phages related to three families, in which ФEEP belonged to the family Siphoviridae. ФEPP phage belonged to Podoviridae and ФEAP belonged to Myoviridae. The ФEAP was specific to acinetobacter baumannii subsequently was chosen to applied on our study. The therapeutic efficacy of ФEAP was evaluated in a mouse model of a complete wound that acquired an infection of A.baumannii. Phage-treated wounds inoculated with ФEAP demonstrated full regeneration; high-quality wound healing, and elimination of bacteria with infection. All of those characteristics combine to ФEAP phage a potential treatment for infections of skin wounds by MDR A.baumannii. Nevertheless, additional investigation and clinical research studies on the phage combinations that are needed to be applied topically in wound infections must be conducted.

Keywords