Optimization of cellulases production under fermintation conditions by halophilic Aspergillus niger

Abd El-Gawad, Assma; Aboalkasem, Nabil; Eweis, M.; Radwan, Tharwat El-Sayed El-Desouky

doi:10.21608/ifjsis.2023.253630.1048

Optimization of cellulases production under fermintation conditions by halophilic Aspergillus niger

Document Type : Original full papers (regular papers)

Authors

¹ Fayoum University, Faulty Of Science, Botany Department

² faculty of science fayoum university

³ CairoUniversity, Faulty Of Science, Botany Department

10.21608/ifjsis.2023.253630.1048

Abstract

A halophilic black Aspergillus niger isolate has showen high cellulolytic activity and was identified by both morphological and molecular identification, and the result was halophilic Aspergillus niger AUMC 15543. The later showed high stability after cultivated for about 12 generations. It was used for cellulases production under submerged fermentation (Smf) conditions. It has been found that optimum temperature and pH yielded maximum cellulases enzymes, dry weight, extracellular protein and soluble sugars production by A. niger were 30°C for culture temperature, and pH5, by yielding maximum dry weight, extracellular protein and soluble sugars of (1170.333 mg/50ml, 1065 mg/ml and 585.333 mg/ml) respectively and maximum titers of cellulases enzymes FPase, CMCase and ß- glucosidase of (1.704 IU/ml, 0.852667 IU/ml and 2.121333 IU/ml) respectively. Culture medium with lactose 1% addition yielded higher titers of FPase, CMCase and ß- glucosidase of (2.106333 IU/ml, 1.111667 IU/ml and 2.615333 IU/ml) respectively and best dry weight, extracellular protein and soluble sugars of (1489.333 mg/50ml, 1376 mg/ml and 886.6667 mg/ml) compared to (control, glucose, maltose and sucrose) respectively.

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